One of the major reasons for studying chemical kinetics is to use measurements of the macroscopic properties of a system, name: Hemin, such as the rate of change in the concentration of reactants or products with time.In a article, mentioned the application of 16009-13-5, Name is Hemin, molecular formula is C34H32ClFeN4O4
Peroxidase Activity of a c-Type Cytochrome b5 in the Non-Native State is Comparable to that of Native Peroxidases
The design of artificial metalloenzymes has achieved tremendous progress, although few designs can achieve catalytic performances comparable to that of native enzymes. Moreover, the structure and function of artificial metalloenzymes in non-native states has rarely been explored. Herein, we found that a c-type cytochrome b5 (Cyt b5), N57C/S71C Cyt b5, with heme covalently attached to the protein matrix through two Cys?heme linkages, adopts a non-native state with an open heme site after guanidine hydrochloride (Gdn?HCl)-induced unfolding, which facilitates H2O2 activation and substrate binding. Stopped-flow kinetic studies further revealed that c-type Cyt b5 in the non-native state exhibited impressive peroxidase activity comparable to that of native peroxidases, such as the most efficient horseradish peroxidase. This study presents an alternative approach to the design of functional artificial metalloenzymes by exploring enzymatic functions in non-native states.
Peroxidase Activity of a c-Type Cytochrome b5 in the Non-Native State is Comparable to that of Native Peroxidases
One of the oldest and most widely used commercial enzyme inhibitors is aspirin, name: Hemin, which selectively inhibits one of the enzymes involved in the synthesis of molecules that trigger inflammation. you can also check out more blogs about 16009-13-5
Reference£º
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion