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Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow-injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG1 or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02mugml-1) and a total assay time of 19min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow-injection analysis, and disposable when necessary. Copyright (C) 2000 Elsevier Science B.V.
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Reference：
Iron Catalysis in Organic Synthesis | Chemical Reviews,
Iron Catalysis in Organic Synthesis: A Critical Assessment of What It Takes To Make This Base Metal a Multitasking Champion